Innovative Methodology In situ dynamically monitoring the proteolytic function of the ubiquitin-proteasome system in cultured cardiac myocytes
نویسندگان
چکیده
Xin Dong,* Jinbao Liu,* Hanqiao Zheng, Joseph W. Glasford, Wei Huang, Quan Hai Chen, Niels R. Harden, Faqian Li, A. Martin Gerdes, and Xuejun Wang Cardiovascular Research Institute and Department of Laboratory Medicine and Pathology, University of South Dakota; Cardiovascular Research Institute, South Dakota Health Research Foundation, Sioux Falls, South Dakota 57105; Department of Pathophysiology, Guangzhou Medical College, Guangzhou, Guangdong; Department of Pathophysiology, Wuhan University, Wuhan, Hubei; and Cardiovascular Research Institute, Nanjing Medical University, Nanjing, Jiangsu, China
منابع مشابه
In situ dynamically monitoring the proteolytic function of the ubiquitin-proteasome system in cultured cardiac myocytes.
The ubiquitin-proteasome system (UPS) is responsible for turnover of most cellular proteins in eukaryotes. Protein degradation by the UPS serves quality control and regulatory functions. Proteasome inhibition showed great promise in effectively treating cancer and restenosis. UPS dysfunction in cardiac hypertrophy and failure has recently been suspected but remains to be investigated. A system ...
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Cardiomyocytes are terminally differentiated cells and thus do not have the ability to dilute damaged proteins and organelles by cell division. Thus, proteolytic and recycling systems within the cardiomyocyte are essential to maintain cardiac function. The major proteolytic systems in the cell are: the ubiquitin–proteasome system, autophagy, and calpain. The ubiquitin–proteasome system degrades...
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Intracellular protein degradation is primarily performed by the ubiquitin-proteasome system (UPS) and the autophagic-lysosomal pathway (ALP). The interplay between these two pathways has been rarely examined in intact animals and the mechanism underlying the interplay remains unclear. Hence, we sought to test in vivo and in vitro the impact of inhibition of the ALP on UPS proteolytic performanc...
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تاریخ انتشار 2004